Bossart, Gregory D.: SEE ALSO Converse et al., 1994; Duignan et al., 1995; Falcón et al., 2003; Miller et al., 2001; Odell et al., 1995; Upton et al., 1989; Walsh & Bossart, 1999; Walsh et al., 1987.
( detail)
x |
|
Upton, Steve J.; Odell, Daniel Keith; Bossart, Gregory D.; Walsh, Michael T.
(detail)
|
| |
|
1989 |
Description of the oocysts of two new species of Eimeria (Apicomplexa: Eimeriidae) from the Florida manatee, Trichechus manatus (Sirenia: Trichechidae).
Jour. Protozool. 36(1): 87-90. 1 tab. 8 figs.
–Describes Eimeria manatus and E. nodulosa, n.spp., from the feces of 9 out of 16 T. m. latirostris examined, and compares them with E. trichechi. The infected manatees included both wild and captive-born specimens, and ones from both coasts of Florida.
|
|
|
Bossart, Gregory D.; Dierauf, Leslie A.
(detail)
|
| |
|
1990 |
Marine mammal clinical laboratory medicine. In: L. A. Dierauf (ed.), CRC handbook of marine mammal medicine: health, disease, rehabilitation.
Boca Raton (Florida), CRC Press, Inc. (735 pp.): 1-52. 10 tabs. 31 figs.
–Discusses techniques of blood sampling (3), hematology (5, 7, 40-41, 43-44), and urine and fecal collection (43, 46) relevant to T. manatus.
|
|
|
Duignan, Pádraig J.; House, Carol; Walsh, Michael T.; Campbell, Terry; Bossart, Gregory D.; Duffy, Noel; Fernandes, Peter J.; Rima, Bert K.; Wright, Scott D.; Geraci, Joseph R.
(detail)
|
| |
|
1995 |
Morbillivirus infection in manatees.
Mar. Mamm. Sci. 11(4): 441-451. 1 tab. 1 fig. Oct. 26, 1995.
|
x |
|
Odell, Daniel Keith; Bossart, Gregory D.; Lowe, Mark T.; Hopkins, Thomas D.
(detail)
|
| |
|
1995 |
Reproduction of the West Indian manatee in captivity. [Abstr.] In: T. J. O'Shea, B. B. Ackerman, & H. F. Percival (eds.), Population biology of the Florida manatee (q.v.).
Information & Technology Rept. (U.S. Dept. Interior, Natl. Biological Service) (vi + 289) 1: 192-193. Aug. 1995.
–An earlier version of this abstr. appeared in O'Shea et al. (1992: 17-18). Notes that length of gestation is still imprecisely known (12-14 months); that females probably mate throughout pregnancy; that spermatogenic activity is low and births rare during December-February; that females who lost calves became pregnant again in a minimum of 2 months; and that birth intervals ranged from 14 to 103 months.
|
x |
|
Bossart, Gregory D.; Baden, Daniel G.; Ewing, Ruth Y.; Roberts, Brenda; Wright, Scott D.
(detail)
|
| |
|
1998 |
Brevetoxicosis in manatees (Trichechus manatus latirostris) from the 1996 epizootic: gross, histologic, and immunohistochemical features.
Toxicologic Pathology 26(2): 276-282. 2 figs. Mar.-Apr. 1998.
–Concludes that brevetoxicosis from exposure to red tide "was a component of and likely played a central role in the 1996 manatee epizootic." Such deaths can result from neurointoxication and/or hemopathy caused by chronic ingestion and/or inhalation of red tide toxins. Unlike the 1982 epizootic, ingestion of ascidians was not a prominent finding in the 1996 necropsies, wherein upper respiratory tract lesions were "the only severe and consistent inflammatory lesions seen".
|
x |
|
Bossart, Gregory D.
(detail)
|
| |
|
1999 |
The Florida manatee: on the verge of extinction?
Jour. Amer. Veter. Med. Assoc. 214(8): 1178-1183. 1 tab. Apr. 15, 1999.
–Detailed gen. acc. of the biology of manatees, threats to their survival in Florida, and medical and other efforts being made on their behalf.
|
|
|
Walsh, Michael T.; Bossart, Gregory D.
(detail)
|
| |
|
1999 |
Manatee medicine. In: M. E. Fowler & R. E. Miller (eds.), Zoo and wild animal medicine: current therapy. 4th Ed.
Philadelphia, W. B. Saunders Co. (xxiii + 747 pp.): 507-516. Illus.
|
|
|
Bossart, Gregory D.
(detail)
|
| |
|
2001 |
Manatees. In: L. A. Dierauf & F. M. D. Gulland (eds.), CRC handbook of marine mammal medicine. Ed. 2.
Boca Raton, etc., CRC Press (lvii + 1063): 939-960. 10 figs.
|
x |
|
Falcón-Matos, Limarie; Mignucci-Giannoni, Antonio A.; Toyos-Gonzáles, Gian M.; Bossart, Gregory D.; Meisner, René A.; Varela, René A.
(detail)
|
| |
|
2003 |
Evidence of a shark attack on a West Indian manatee (Trichechus manatus) in Puerto Rico.
Jour. Neotropical Mammalogy (Mastozoologia Neotropical) 10(1): 6-11. 1 fig. Jan.-June 2003.
–Spanish summ. Reports healed scars from a bite of a large shark (probably a tiger shark, Galeocerdo cuvier) on an adult female manatee that died of intestinal impaction and respiratory distress of uncertain relation to the shark bite. This is the first confirmed report of an ante-mortem shark attack on a manatee.
|
|
x |
|
Flewelling, Leanne J.; Naar, Jerome P.; Abbott, Jay P.; Baden, Daniel G.; Barros, Nélio B.; Bossart, Gregory D.; Bottein, Marie-Yasmine D.; Hammond, Daniel G.; Haubold, Elsa M.; Heil, Cynthia A.; Henry, Michael S.; Jacocks, Henry M.; Leighfield, Tod A.; Pierce, Richard H.; Pitchford, Thomas D.; Rommel, Sentiel A.; Scott, Paula S.; Steidinger, Karen A.; Truby, Earnest W.; Van Dolah, Frances M.; Landsberg, Jan H.
(detail)
|
| |
|
2005 |
Red tides and marine mammal mortalities.
Nature 435(7043): 755-756. 2 figs.
|
|
|
Bossart, Gregory D.; Mignucci-Giannoni, Antonio A.; Rivera-Guzman, Antonio L.; Jimenez-Marrero, Nilda M.; Camus, Alvin C.; Bonde, Robert K.; Dubey, Jitender P.; Reif, John S.
(detail)
|
| |
|
2012 |
Disseminated toxoplasmosis in Antillean manatees (Trichechus manatus manatus) from Puerto Rico.
Diseases of Aquatic Organisms 101: 139-144. 3 figs. doi: 10.3354/dao02526 Nov. 8, 2012.
–Available at: http://www.int-res.com/abstracts/dao/v101/n2/p139-144/
ABSTRACT: Necropsies were conducted on 4 Antillean manatees Trichechus manatus manatus that were stranded in single events on the coastal beaches of Puerto Rico from August 2010 to August 2011. Three manatees were emaciated and the gastrointestinal tracts were devoid of digesta. Microscopically, all manatees had severe widespread inflammatory lesions of the gastro-intestinal tract and heart with intralesional tachyzoites consistent with Toxoplasma gondii identified by histological, ultrastructural and immunohistochemical techniques. The gastrointestinal lesions included severe, multifocal to diffuse, chronic-active enteritis, colitis and/or gastritis often with associated ulceration, necrosis and hemorrhage. Enteric leiomyositis was severe and locally extensive in all cases and associated with the most frequently observed intralesional protozoans. Moderate to severe, multifocal, chronic to chronic-active, necrotizing myocarditis was also present in all cases. Additionally, less consistent inflammatory lesions occurred in the liver, lung and a mesenteric lymph node and were associated with fewer tachyzoites. Sera (n = 30) collected from free-ranging and captive Puerto Rican manatees and a rehabilitated/released Puerto Rican manatee from 2003 to 2012 were tested for antibodies for T. gondii. A positive T. gondii antibody titer was found in 2004 in 1 (3%) of the free-ranging cases tested. Disease caused by T. gondii is rare in manatees. This is the first report of toxoplasmosis in Antillean manatees from Puerto Rico. Additionally, these are the first reported cases of disseminated toxoplasmosis in any sirenian. The documentation of 4 cases of toxoplasmosis within one year and the extremely low seroprevalence to T. gondii suggest that toxoplasmosis may be an emerging disease in Antillean manatees from Puerto Rico.
|
|
|
Guerra Neto, Guilherme; Galvão Bueno, Marina; Silveira Silva, Rodrigo Otavio; Faria Lobato, Francisco Carlos; Plácido Guimarães, Juliana; Bossart, Gregory D.; Marmontel, Miriam
(detail)
|
| |
|
2016 |
Acute necrotizing colitis with pneumatosis intestinalis in an Amazonian manatee calf.
Diseases of Aquatic Organisms 120: 189-194. 2 figs. doi: 10.3354/dao03019. Aug. 9, 2016.
–ABSTRACT: On 25 January 2014, a 1 mo old female Amazonian manatee Trichechus inunguis calf weighing 12 kg was rescued by air transport in Guajará, Brazil, and transferred to Mamirauá Institute's Community-based Amazonian Manatee Rehabilitation Center. The calf presented piercing/cutting lesions on the back, neck, and head, in addition to dehydration and intermittent involuntary buoyancy. X-ray analysis revealed a large amount of gases in the gastrointestinal tract. Daily procedures included wound cleaning and dressing, clinical and laboratory monitoring, treatment for intestinal tympanism, and artificial feeding. Adaptation to the nursing formula included 2 kinds of whole milk. Up to 20 d post-rescue the calf presented appetite, was active, and gained weight progressively. Past this period the calf started losing weight and presented constant involuntary buoyancy and died after 41 d in rehabilitation. The major findings at necropsy were pneumatosis intestinalis in cecum and colon, pulmonary edema, and hepatomegaly. The microscopic examination revealed pyogranulomatous and necrohemohrragic colitis with multinucleated giant cells, acute multifocal lymphadenitis with lymphoid depletion in cortical and paramedullary regions of mesenteric lymph nodes, and diffuse severe acinar atrophy of the pancreas. Anaerobic cultures of fragments of cecum and colon revealed colonies genotyped as Clostridium perfringens type A. We speculate that compromised immunity, thermoregulatory failure, and intolerance to artificial diet may have been contributing factors to the infection, leading to enterotoxemia and death.
|
|
|
Zahin, Maryam; Dean, William L.; Ghim, Shin-je; Joh, Joongho; Gray, Robert D.; Khanal, Sujita; Bossart, Gregory D.; Mignucci-Giannoni, Antonio A.; Rouchka, Eric C.; Jenson, Alfred B.; Trent, John O.; Chaires, Jonathan B.; Chariker, Julia H.
(detail)
|
| |
|
2018 |
Identification of G-quadruplex forming sequences in three manatee papillomaviruses.
PLoS One 13(4): e0195625. 5 tabs. 6 figs. + online supplementary material. doi:10.1371/journal.pone.0195625 April 9, 2018.
–ABSTRACT: The Florida manatee (Trichechus manatus latirotris) is a threatened aquatic mammal in United States coastal waters. Over the past decade, the appearance of papillomavirus-induced lesions and viral papillomatosis in manatees has been a concern for those involved in the management and rehabilitation of this species. To date, three manatee papillomaviruses (TmPVs) have been identified in Florida manatees, one forming cutaneous lesions (TmPV1) and two forming genital lesions (TmPV3 and TmPV4). We identified DNA sequences with the potential to form G-quadruplex structures (G4) across the three genomes. G4 were located on both DNA strands and across coding and non-coding regions on all TmPVs, offering multiple targets for viral control. Although G4 have been identified in several viral genomes, including human PVs, most research has focused on canonical structures comprised of three G-tetrads. In contrast, the vast majority of sequences we identified would allow the formation of non-canonical structures with only two G-tetrads. Our biophysical analysis confirmed the formation of G4 with parallel topology in three such sequences from the E2 region. Two of the structures appear comprised of multiple stacked two G-tetrad structures, perhaps serving to increase structural stability. Computational analysis demonstrated enrichment of G4 sequences on all TmPVs on the reverse strand in the E2/E4 region and on both strands in the L2 region. Several G4 sequences occurred at similar regional locations on all PVs, most notably on the reverse strand in the E2 region. In other cases, G4 were identified at similar regional locations only on PVs forming genital lesions. On all TmPVs, G4 sequences were located in the non-coding region near putative E2 binding sites. Together, these findings suggest that G4 are possible regulatory elements in TmPVs.
|
|
Bibliography and Index of the Sirenia and Desmostylia 