Bibliography and Index of the Sirenia and Desmostylia  

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"Linedale, Richard"

 
 
 Wong, Arthur; Lanyon, Janet M.; McKee, Sara J.; Linedale, Richard; Woolford, Lucy; Long, Trevor; Leggatt, Graham R. (detail)
   
2018
Development of a polyclonal anti-dugong immunoglobulin G (IgG) antibody with evaluation of total plasma IgG in a living dugong (Dugong dugon) population.
Veterinary Immunology & Immunopathology 200: 16-25. 2 tabs. 4 figs. DOI: 10.1016/j.vetimm.2018.04.003 Apr. 8, 2018.
–ABSTRACT: Species-specific antibodies (Ab) for the measurement of immunoglobulins (Ig) are valuable tools for determining the humoral immune status of threatened and endangered wildlife species such as dugongs. However, no studies have reported antibody reagents against dugong immunoglobulin. The object of this study was to develop an Ab with specificity for dugong IgG and apply this tool to survey total IgG levels in plasma samples from a live wild population of dugongs in southern Queensland, Australia. Dugong IgG was isolated from plasma by protein A/G column chromatography and a polyclonal antiserum was successfully raised against the dugong IgG through immunization of mice. The anti-dugong antiserum was reactive with dugong serum but not immunoglobulin from other species such as rats and humans. When tested against a panel of dugong plasma samples, relative IgG levels from dugongs (n?=?116) showed biologically relevant relationships with pregnancy status and a principal component of Body Mass Index (BMI)/globulin/fecal glucocorticosteroid (chronic stress) levels combined, which together accounted for 9.2% of the variation in total Ig levels. Together these data suggest that dugongs show variation in total IgG and that this correlates with some physiological parameters of dugong health.
 
 
 Wong, Arthur; Lanyon, Janet M.; O'Handley, Ryan; Linedale, Richard; Woolford, Lucy; Long, Trevor; Leggatt, Graham R. (detail)
   
2019
Serum antibodies against Toxoplasma gondii and Neospora caninum in southeast Queensland dugongs.
Mar. Mamm. Sci. https://doi.org/10.1111/mms.12629. Jan. 2020; publ. online June 24, 2019.
–ABSTRACT: The dugong (Dugong dugon) is an herbivorous marine mammal that inhabits tropical inshore waters and thus may be vulnerable to pollutants and terrestrial pathogens as a result of coastal runoff. In this study, serum samples collected from live, wild dugongs (n = 114) in an embayment located on the urbanized southeast Queensland coast of Australia during 2008–2014, were measured for IgG antibody levels specific to Toxoplasma gondii and Neospora caninum. An ELISA used to measure T. gondii tachyzoite antibodies indicated a non-Gaussian distribution of antibody level, with five dugongs identified as high outliers. Mean levels of antibodies specific for T. gondii in dugongs sampled in 2014 were significantly higher than in 2010 (p=.006) and 2011 (p=.009) with an elevation in mean antibody levels after a major 2011 flood event relative to antibody levels prior to the flood (p<.0001). A competitive ELISA to detect N. caninum antibody indicated a normal distribution of antibody with no high outliers. Mean antibody level for N. caninum was highest in 2012 and declined significantly in 2014 (p=.004). This is the first survey of antibodies directed against T. gondii and N. caninum in dugongs and suggests future health monitoring of this species.
Daryl P. Domning, Research Associate, Department of Paleobiology, National Museum of Natural History, Smithsonian Institution, Washington, D.C. 20560, and Laboratory of Evolutionary Biology, Department of Anatomy, College of Medicine, Howard University, Washington, D.C. 20059.
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