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"Drummond de Mello, D"

 
 
 Drummond de Mello, Daniela Magalhães; Silva, Vera Maria Ferreira da; Rosas, Fernando César Weber (detail)
   
2011
Serum biochemical analytes in captive Amazonian manatees (Trichechus inunguis).
Veterinary Clinical Pathology 40(1): 74-77. 1 tab. DOI:10.1111/j.1939-165X.2011.00297.x March 2011.
–ABSTRACT: Background: Establishment of reference values for serum biochemical analytes is important for monitoring health and physiological status of captive animals.
 Objective: The purpose of this study was to measure and report ranges for serum biochemical analytes in Amazonian manatees (Trichechus inunguis).
 Methods: Blood samples were collected from 24 healthy captive Amazonian manatees that comprised a mixture of adults, subadults, and calves and males and females; serum analytes were measured and analyzed using a dry reagent bench-top chemical analyzer. Comparisons were made between sexes and with previously published values of closely related species.
 Results: Medians and ranges (minimum–maximum) of values for the analytes were: lactate dehydrogenase (LDH), 151 (111–278) U/L (n=20); creatine kinase, 144 (76–315) U/L (n=11); alanine aminotransferase, 10 (2–28) U/L (n=18); aspartate aminotransferase, 14 (5–28) U/L (n=21); ?-glutamyltransferase, 47 (36–73) U/L (n=21); amylase, 1428 (1010–1874) U/L (n=21); alkaline phosphatase, 73 (36–141) U/L (n=19); total protein, 6.8 (6.2–8.0) g/dL (n=24); albumin, 3.3 (2.6–4.1) g/dL (n=21); cholesterol, 188 (101–399) mg/dL (n=21); triglycerides, 126 (60–236) mg/dL (n=21); glucose, 47 (22–69) mg/dL (n=21); urea, 43 (21–69) mg/dL (n=21); uric acid, 1.1 (0.5–1.8) mg/dL (n=22); creatinine, 2.2 (1.5–3.3) mg/dL (n=22); total bilirubin, 0.2 (0.2–2.0) mg/dL (n=21); calcium, 12.7 (10.2–18.6) mg/dL (n=24); iron, 282 (207–457) ?g/dL (n=13); and magnesium, 6.9 (4.3–8.9) mg/dL (n=20). With the exception of LDH, no differences were observed between sexes.
 Conclusions: The ranges obtained in this study provide important preliminary estimates for concentrations and activities of serum analytes in Amazonian manatees until a larger reference interval study can be conducted.
Daryl P. Domning, Research Associate, Department of Paleobiology, National Museum of Natural History, Smithsonian Institution, Washington, D.C. 20560, and Laboratory of Evolutionary Biology, Department of Anatomy, College of Medicine, Howard University, Washington, D.C. 20059.
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